Physiological Characterization of a Bacillus licheniformis Strain in Chemostat Cultivations

The aim of the current work was to collect relevant data on the physiology of Bacillus licheniformis in chemostat cultivations. The first experiments have shown some nutrient limitation other than the carbon source in the culture medium. Besides, the growth yields observed were much smaller than expected for bacteria growing in glucose. As a consequence of the abovementioned results, the adaptation of the original culture medium to a suitable one was set as the latest objective of this work. Further experiments were developed in order to test the behavior of the microorganism under lower glucose concentrations and higher trace metal concentration. The results have shown that these adjustments were not sufficient to circumvent the nutrient limitation issue.